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rabbit anti phospho mlc ii  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti phospho mlc ii
    Rabbit Anti Phospho Mlc Ii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+myosin+light+chain+mlc/Phospho-Myosin+Light+Chain+2+(Thr18%2FSer19)+Antibody/pmc12922757-243-44-49
    Average 96 stars, based on 777 article reviews
    rabbit anti phospho mlc ii - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: INTESTINAL EPITHELIAL-SPECIFIC OCCLUDIN DELETION WORSENS GUT PERMEABILITY AND SURVIVAL FOLLOWING SEPSIS
    Article Snippet: Samples were separated on Mini-PROTEAN TGX Stain Free Precast Gels (Bio-Rad) at 160 V for 50 min, and proteins were activated on the ChemiDoc Touch MP Imaging System (Bio-Rad) for 5 min. Proteins were then blotted onto polyvinylidene difluoride membranes (Bio-Rad) using a semiwet method via Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were blocked with 3% (w/v) bovine serum albumin or 3% (w/v) nonfat dry milk in TBS buffer for 1 h at room temperature, followed by incubation at 4°C overnight with the following primary antibodies: rabbit polyclonal anti-occludin (1:500, 71–1500, Thermo Fisher Scientific), rabbit polyclonal anti-ZO-1 (1:500, 61–7300, Thermo Fisher Scientific), rabbit polyclonal anti-tricellulin (1:500, 48–8400, Thermo Fisher Scientific), rabbit polyclonal anti-JAM-A (1:1,000, 36–1700, Thermo Fisher Scientific), mouse monoclonal anti-phospho-myosin light chain (pMLC) (1:1,000, 3675, Cell Signaling Technology), rabbit anti-myosin light chain (MLC) (1:1,000, 3672, Cell Signaling Technology), mouse monoclonal anti-claudin 2 (1:1,000, 32–5600, Thermo Fisher Scientific), rabbit polyclonal anti-claudin 4 (1:500, 36–4800, Thermo Fisher Scientific), rabbit polyclonal anti-claudin 5 (1:2,000, 34–1600, Thermo Fisher Scientific), rabbit polyclonal anti-claudin 8 (1:2,000, 40–0700Z, Thermo Fisher Scientific), rabbit polyclonal anti-claudin 12 (1:500, 38–8200, Thermo Fisher Scientific), rabbit polyclonal anti-claudin 15 (1:500, 38–9200, Thermo Fisher Scientific), rabbit polyclonal anti-Bax (1:1,000, 2772, Cell Signaling Technology, Danvers, MA), rabbit polyclonal anti-Bcl-2 (1:1,000, 2876, Cell Signaling Technology), rabbit polyclonal anti-cleaved capase-3 (1:1,000, 9661, Cell Signaling Technology), and rabbit polyclonal anti-β-actin (1:1,000, 4967, Cell Signaling Technology). .. Membranes were then washed and incubated with horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (1:2,000, Cell Signaling Technology) secondary antibodies for 1 h. Blots were developed with Clarity ECL Western Blotting Substrates (Bio-Rad) and visualized with ChemiDoc Touch MP Imaging System.



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